RESUMO
Objective: The lignan components in Schizandrae Fructus residue extracts were analized and evaluated. The protective effect of Schizandrae Fructus residue extracts on acute-hepatic damnification rat and the influence of that on intestinal flora were researched in the process of Shengmai Injection production, in order to provide the scientific basis for the development and utilization of Schizandrae Fructus extract. Methods: Schizandrae Fructus residue extract was extracted by the 60% ethanol. The model of acute-hepatic damnification rats induced by CCl4 was prepared, the protective effects of Schizandrae Fructus residue extracts on hepatic-damnification rats were evaluated through the data of ALT, AST, ALB, and TP; The function of the extracts were evaluated by histopathological observation of the liver and colon of rats; Effect of extracts on hepatic-damnification rat's intestinal flora was evaluated by the number and the number variation of the intestinal flora. Results: The results showed that the mass fractions of schisandrin A, schizandrin B, schizandrin C, gomisin A, gomisin B, and schisantherin A were 1.44, 3.42, 1.68, 4.22, 2.92, and 0.58 mg/g, respectively. Pharmacodynamic evaluation showed that Schizandrae Fructus extracts can significantly reduce the content of AST and ALT (P < 0.01) and increase the content of ALB and TP in a certain extent in the serum of acute-hepatic damnification rats (P < 0.05); Mucous damage score of liver and colon is significantly decreased; Compared to liver injury group, Schizandrae Fructus extracts of high dose group and low dose group can significantly promote the proliferation of Lactobacillus and Bifidobacterium (P < 0.05) but reduce the number of Enterococcus and Enterobacteria in the intestinal tract of rats (P < 0.05). Conclusion: Schizandrae Fructus residue extracts have the protective effects on the acute-hepatic damnification rats induced by CCl4, the effect of regulating intestinal flora and the proliferation of probiotics, the effect of protecting the constitution integrity of colon tissue, which provide a main basis for its further development and utilization.
RESUMO
Objective: Multi-type resource chemical constituents in Schizandrae Fructus residues were analyzed in the process of Shengmai Injection production, in order to provide the scientific basis for Schizandrae Fructus in the further process of industrialization. Methods: The lignan components were analysed and evaluated by HPLC-UV method. After using NaOH to extract sample, the BCA method was adopted to measure the mass fraction of total protein and take bovine serum albumin as the reference. Using UV-Vis spectrophotometer to measure the mass fraction and constitutes of neutral polysaccharide and acidic polysaccharide, respectively. The equipment for raw fiber determination was taken to gauge the crude fiber content of Schizandrae Fructus residues. Results: The mass fractions of schisandrin A, schizandrin B, schizandrin C, gomisin A, gomisin B, and schisantherin A were 1.442 4, 3.788 0, 1.350 9, 4.399 3, 3.231 3, and 0.505 3 mg/g, respectively. Compared with the original medicinal materials, the technology utilization rate of gomisin A was 20.84% during the process of Shengmai Injection production. But the gomisin B virtually was unused and remained in the residues. The mass fractions of schisandrin A, schizandrin B, schizandrin C, and schisantherin A were higher than that of the original medicinal material. The available macromolecular substances are proteins, polysaccharide, and crude fiber. The content of total proteins was 14.69%. The mass fractions of neutral polysaccharide and acidic polysaccharide were 3.82% and 1.31%, respectively. The analysis on crude fiber showed that the mass fraction of crude fiber in Schizandrae Fructus residues was 43.80%. Conclusion: The analysis shows that Schizandrae Fructus residues contain many resource chemical components in the process of Shengmai Injection production, such as lignins, protein, saccharides, and crude fiber. The strategy for recycling and possible way is proposed exploringly based on the available resource chemical components of Schizandrae Fructus residues and water-extracting technology. It provides the reference for the utilization of waste resource in further process of industralization, promoting the resource conservation, and development of environmental protection, realizing the harmonious coexistence between the economy and ecology.